Gs‐DREADD Regulation of ENaC

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Landscape of ENaC regulation in the kidney.

AS THE REAL ESTATE MAXIM GOES, “There are three things that matter in property: location, location, location.” The same could be said about epithelial Na channel (ENaC) regulation in the mouse kidney. ENaC is an heteromeric ion channel (comprised of -, -, and -subunits) expressed in the aldosterone-sensitive distal nephron (ASDN) and provides the final conduit for Na reabsorption from the tubul...

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Nongenomic regulation of ENaC by aldosterone.

Aldosterone is involved in salt and water homeostasis. The main effect is thought to involve genomic mechanisms. However, the existence of plasma membrane steroid receptors has been postulated. We used whole cell patch clamp to test the hypothesis that epithelial sodium channels (ENaC) expressed by renal collecting duct principal cells can be regulated nongenomically by aldosterone. In freshly ...

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Regulation of ENaC trafficking in rat kidney

The epithelial Na channel (ENaC) forms a pathway for Na(+) reabsorption in the distal nephron, and regulation of these channels is essential for salt homeostasis. In the rat kidney, ENaC subunits reached the plasma membrane in both immature and fully processed forms, the latter defined by either endoglycosidase H-insensitive glycosylation or proteolytic cleavage. Animals adapted to a low-salt d...

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Noncoordinate regulation of ENaC: paradigm lost?

The epithelial sodium channel (ENaC) is composed of the three homologous subunits alpha, beta, and gamma. The basic oligomerization process inferred from all studies in heterologous systems is preferential assembly of the three subunits into a single oligomeric form. However, there is also considerable evidence that channels composed of only alpha-, alphabeta-, or alphagamma-subunits can form u...

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BACKGROUND The importance of beta and gamma epithelial Na(+) channel (ENaC) proteins in vascular smooth muscle cell (VSMC)-mediated pressure-induced constriction in renal interlobar arteries has been demonstrated recently. In renal epithelial tissue, ENaC expression is regulated by angiotensin II (Ang II). However, whether Ang II regulates vascular ENaC expression has never been determined. The...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2021

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fasebj.2021.35.s1.03570